الفهرس | Only 14 pages are availabe for public view |
Abstract SUlvIMA RY Fenugreek seeds of both Giza 30 and Family 77 were g,crminated in derk for 120 hr,3at 25°C. The effect of germination on the chemical composition, carbohYdrates, proteins, protease activity and trypsin inhibitor actiivity in the seeds germinated for 24, 48, 72 , 96 and 120 hrs ’.’:erestudi~d, :unsoaked and ungerm:[11E\ted seeds served ’ ’ 8S control. Amino acid composition of ungermin~ted and germinated seeds for 120 hr was also performed. The pH solubility profiles for total albumins and total globuulins extfacted from ungerminated and germinated (120 hr) oeeds were inveotigated. The results could be summarised 1)0- Crude protein increased from 26.5 to 32.8 and from 25.9 to 30.9 % during the first 48 brsof germinating seeds of Giza 30 and Family 77 respectively. As germination advanced, further changes in crude pro- b- Germination increased crude fiber and crude ash but decreased crude fat by 25.3 and 37.4 % of the ori- ginal amount in germinating GizQ 30 and Family 77 c- Germination slightly increased the P but decreased K in both fenuereek snmples. 2)a- The starch content was higher in the ungerminated seeds of Family 77 (22.13 %) than Giza 30 (19.18%). It decreased to 51.73 and 63.97 % of its original amounts in Family 77 and Giza 30 seeds respectively by the end of germination period. b- The total soluble, total reducing and total non reducing sugars increased during germination. c- Pentos~n content was higher in the ungerminated seeds of Giza 30 (0.63 %) than Family 17 (0.38 %). Germination for 120 hrsincreased pentosan by 14.3 and 65.7 % in Giza 30 and Family seeds respectively. Pentoses decreased gradually during germination. d- Paper chromatographic examination of 80 % ethanol extracts of fenugreek samples re¥Ba~ed the presence of slucose, fructose, sucrose and two members of raffinose Family (raffinose and stachyose) at all stages of germination period. e- Ungerminated seeds of GjNr 10 and Family 77 contaained 0.4 and 0.32 % raffinose respectively, which gradually decreased to 35 and 37.5 % of its amounts by the end of the germination period. f- Stachyose accounted for 0.28 and 0.24 % in the ungerminated seeds of Giza 30 ano Family 77 respectively. This sugar decreased to 39.2 and 8.2 % of its original amount in Giza 30 and Family 77 seeds after germination for 120 hrs. 3)a- With the exception of sulfur containing amino acids and valine, the levels of the essential a~ino acids in germinated fenugreek seeds· meet or exceed the FAO/WHO (1973) recommendation. h- The S-contnining amino acids and histidine inncreased by 45.4, 26.6 , 40.7 and 28 % in ger~inated seeds of Giza 30 and Family 77 respectively. A narked increase in phenylalanine (20 to 23.8 %) and tryptoophan (15.3 to 16.6 %) was observed after 120 hr gerrmination. c- Slight chances in some other essential and nonnessential amino acid were found. 4)8- Albumins decreased from 43 to 28 % and 48 to 11 % of the total extractable proteins of Giza 30 and Family 77 after 120 hrsgermination . b- The globulins which made 29.5 and 30 % of the total extractable proteins in unge~ninated see~s of Giza 30 and Family 77 showed si:ni-lar .tre.nd during ger- ;:) i r’l ~ t fan.’ |