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Abstract
Rift Valley Fever VIrus is an arthropod borne VIrus transmitted by
mosquitoes that infect human and animals. RVFV induce almost 100% mortality
among young animals and high rate of abortion in pregnant females. In humans
infection can vary from mild to very sever clinical picture, including temporary
and definitive blundness, encephalitis and hemorrhagic fever syndrome and
hepatitis with a fatal outcome. The corner stone of virus control is eradication of
mosquitoes and vaccination and recently have control by antiviral drugs so in
this study we study effect of amantadine hydrochloride and acyclovir on RVFV
reduction-or prevention in vitro (in BHK.-21 cell) and in vivo in (baby mice).
In this study we evaluate the effect of amantadine hydrochloride toxicity on
BHK.-21 cells and baby mice (5 - 15 gm) and found that amantadine has safe
effect on cell culture from 25 ug/ml to 500 ug/ml and have toxicity effect on
BHK.-21 cell at 1000 ug/ml till 5000 ug/ml and not have toxic effect in mice till
5000 ug/ml.
. The effective dose of amantadine hydrochloride in vitro ranged from 100
ug /ml to 500 ug/ml give reduction effect to infected BHK.-21 cell when
inoculated with or before inoculation with RVFV and have not effect after
inoculation of RVFV, but in mice (5 - 15 gm) has in vivo treatment use by high
dose as dose of treat of 1000 ug/rnl to 5000 ug/rnl with or after or before
inoculation with RVFV.
To evaluate the effect of AB on RVFV by titration test and lab in tissue
culture (BHK.-21 cells), 25 ug/ml and 50 ug/rnl dose not stop RVFV at treat
before or after or with RVFV infection. But doses from 100 ug/rnl to 500 ug/ml
stop RVFV at injection before or with RVFV infection but at treat after infection
not stop virus and this measure by reduction rate and found the reduction is