الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of the work is to find simple and reproducible assay to be used clinically to decide on a real time basis (a) which patient experience an overshoot of circulating haematopoietic progenitors of relevant magnitude for considering the large-scale collection of these calls by leukapheresis, (b) when leukapheresis should be performed, and (c) how many consecutive leukapheresis procedures are necessary to harvest quantities of haematopoietic progenitor cells sufficient for successful transplantation. In conclusion, our results support the feasibility of flow kilometric enumeration of CD34+/CD38+ cells. flow cytometry may be as predictive as CFU analysis and will allow the infusion of a known number of progenitor cells. this observation, however, could be applied for the PBSC aphaeresis product and breakdown when these progenitors are derived from the bone marrow. The flow cytometric determination of CD34+population has not been proven to be better predictor of peripheral blood stem cell performance, as well as, the marrow repopulating ability than has the CFU-GM assay .lt is not, however, proven to be Worse. Its rapid results and relative standardization confirmed the implementation of this assay as an addition to the CF-GM assay. |