الفهرس 
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Abstract
The incidence of Salmonella in Egypt was estimated using ISO 6579 (2002)
which has detection limit of 100 cfu/25 g and 100% relative accuracy and was
compared with polymerase chain reaction. A polymerase chain reaction assay
was developed for the generic detection of Salmonella spp. and the
identification of S. Enteritidis, S. Gallinarum, S. Pullorum and S. Typhimurium
from poultry related samples. Specificity of the assay was tested using 7
Salmonella standard strains and 3 non Salmonella standard strains and 13
isolated Salmonella serovars. The assay was 100% specific for Salmonella
detection. At the genus level, peR-RV detected 2 more positive field samples
than the standard microbiological techniques and results were obtained faster.
A multiplex polymerase chain reaction assay was used for simultaneous
detection of S. Enteritidis, S. Gallinarum, S. Pullorum and S. Typhimurium
from poultry related samples.