الفهرس 
Chapter I : IntroductionOnly 14 pages are availabe for public view
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Abstract
6. Rift valley fever (RVF) is an acute or peracute viral, infection and highly fatal zoonotic disease. It affects many animal species with high economic losses. It is transmited to human begins and has great importance in public health.
A serosurvey investigation was carried out to study the situation of Rift Valley Fever disease in different animals and different governorates in Egypt during (2010–2011). Serum samples collected from sheep, goats, buffaloes and cattle vaccinated with RVF in-activated vaccine as well as from sheep, goat, cattle, buffaloes and camels of non-vaccinated animals in different seasons from different governorates (El-Qalyubia, El-Dakahlia, El-Sharqia, Kafr El-Sheikh, Marsa Matruh and El-Monufia) to study the effect of the vaccine on vaccinated animals and detection of anti-bodies from non-vaccinated animals with trails for detection of viral antigen. Rats were collected for detection of viral antigen or RNA in tissue of rats was carried out.
The serological techniques used in this study were AGPT, SNT and ELISA.
In our study the results of AGPT on serum sample collected from vaccinated animals from El-Qalyubia, Marsa Matruh and El-Monufia governorates were 8.88%, 80% and 68% respectively, the results of AGPT on serum sample collected from non-vaccinated animals from Qualubya, El-Dakahlia, El-Sharqia and Kafr El-Sheikh governorates were 2.85%, 14%, 24% and 10% respectively. While the results of SNT on serum sample collected from vaccinated animals from El-Qalyubia, Marsa Matruh and El-Monufia governorates were 17.77%, 94% and 80% respectively, the results of SNT on serum sample collected from non-vaccinated animals from El-Qalubyia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh governorates were 4.76%, 19%, 30% and 15% respectively.
In our study the results of ELISA on serum samples collected from vaccinated animals from El-Qalyubia, Marsa Matruh and El-Monufia governorates which were 16.66%, 93% and 79% respectively, the results of ELISA on serum samples collected from non-vaccinated animals from Qualubya, El-Dakahlia, El-Sharqia and Kafr El-Sheikh governorates which were 4.52%, 18.5%, 29.5% and 14.5% respectively.
The results of virus isolation from animal serum samples on tissue culture revealed that there is no circulating virus in the collected serum samples from the six governorates during the period of study
Results of RT- PCR and real time PCR on sera samples and inoculated cell culture collected from El-Sharqia and Kafr El-Sheikh governorates for detection of viral RNA were negative. This indicates there is no circulating virus in the examined serum samples and cell culture collected from animals in these governorates during period of study.
Current study indicated that the results of virus isolation from liver suspension of rats on tissue culture revealed there is no characteristic CPE of RVF so no circulating virus in the collected serum samples from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh governorates during the period of study.
In our study the results of RT-PCR and real time PCR on liver suspension of rats collected from El-Qalyubia, El-Dakahlia, El-Sharqia and Kafr El-Sheikh governorates for detection of viral RNA were negative. This indicates there is no circulating virus in the examined liver suspension samples collected from animals in these governorates during period of study
from the obtained results we recommended vaccination of the susceptible animals in all governorates by RVF in-activated vaccine with good supervision provided by general organization of veterinary services on application of vaccine.