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Abstract The present work was performed to study the possible protective role of green tea and curcumin as an antioxidants and free radical scavengers against changes induced by 2, 3, 7, 8- Tetrachlorodibenzo-p-dioxin (TCDD) administration to male Swiss albino mice. In this study, serum protein fraction, differential white blood cells, AhR level, CYP450 level, P53 level, CAT activity, SOD activity and GSH concentration as well as body weight level and the histological changes in the liver, kidneys, spleen and testes were studied. The present work included two experiments: Experiment I The mice were randomly divided into seven groups, 20 mice for each: · The first group: served as a control where mice were given oral dose of vehicles only. · The second group: served as a control where mice were given oral dose with tween-80 only. · The third group: served as a control where mice were given oral dose with corn oil only. · The fourth group: designated TCDD, where mice were given oral dose of TCDD (1.332 mg/kg b.w). · The fifth group: designated GT and TCDD, where mice were given oral dose of green tea (0.5mg/kg b.w) 30 min before the administration TCDD. · The sixth group: designated Cur. and TCDD, where mice were given oral dose of curcumin (0.5mg/kg b.w) 30 min before the administration TCDD. · The seventh group: designated of combined dose of GT, Cur. + TCDD, where mice were given oral dose of green tea & curcumin (1 mg/kg b.w) 30 min before the administration TCDD. All the above doses were repeated daily for 30 days. Experiment II Eighty mice were given daily oral doses of TCDD (1.332 mg/kg b.w). After 30 days of TCDD administration, the mice were divided into four subgroups, 20 mice each. · The first subgroup: designated (Recov), was left for recovery without any type of treatment for 30 days. · The second subgroup: designated (Recov + GT), where mice were given daily oral dose of green tea (0.5 mg/kg b.w) for 30 days. · The third subgroup: designated (Recov + Cur.) where mice were given daily oral dose of curcumin (0.5 mg/kg b.w) for 30 days. · The fourth subgroup: designated (Recov +GT+ Cur.) where mice were given combined oral dose of green tea & curcumin daily (1 mg/kg b.w) for 30 days. The main results obtained from this study are summarized as the following: · TCDD administration greatly increased AhR level, CYP450 level in the liver homogenates versus those of control animals. · TCDD administration greatly decreased P53 level in the liver homogenates versus those of control animals. · TCDD administration greatly decreased the levels of IgG light & heavy chain, antitrypsin and albumin of blood serum in different treated mice versus those of control animals.· TCDD administration greatly increased transferrin, haptoglopin and acid glycoprotein levels of blood serum in different treated mice versus those of control animals. · TCDD administration greatly decreased total white blood cells count (WBC); lymphocytes, eosinophils, monocytes and basophiles levels where it increased level of neutrophils of blood serum in different treated mice versus those of control animals. · TCDD administration greatly inhibited CAT, SOD activites and GSH concentration in the liver, kidneys, spleen and testes homogenates versus those of control animals. · TCDD administration greatly decreased body weight in different treated animals versus those of control animals. · Green tea & curcumin exerted a powerful inhibitory effect on the the levels of AhR and CYP450 in the examined tissues compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful stimulatory effect on the level of P53 in the examined tissues compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful inhibitory effect on the increased levels of transferrin, haptoglopin and acid glycoprotein levels of blood serum compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful stimulatory effect on the levels of IgG light & heavy chain, antitrypsin and albumin of blood serum compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful stimulatory effect on the level of total white blood cells count (WBC); lymphocytes, eosinophils, monocytes and basophiles levels and they were found to decrease the level of neutrophils of blood serum compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful stimulatory effect on the activities of CAT and SOD as well as restoration of the concentration of GSH in the examined tissues compared to those subjected to TCDD administration. · Green tea & curcumin exerted a powerful stimulatory effect on the level of body weight in different treated animals compared to those subjected to TCDD administration. · The combined doses of (GT+Cur.) were found to increase the levels of P53, IgG light & heavy chain, transferrin, albumin, total white blood cells count (WBC); lymphocyts, eosinophils, monocytes, basophiles levels; activity of CAT, SOD and GSH concentration in TCDD-exposed mice of experiment I and II compared with curcumin. · TCDD-administration markedly induced severe histological changes in the liver, kidney, spleen and testes as well as the fine structures of the liver compared with control animals. · TCDD-administration markedly increased the amount of collagenous fibers in the liver, kidney, spleen and testes versus to those of control mice. · TCDD-administration reduced the amount of protein in the liver and kidney versus to those of control mice. · TCDD-administration reduced the amount of carbohydrate materials in the hepatic, renal and splenic tissues versus the contents of control animals. · Green tea & curcumin proved to have strong protective effect in the prevention of TCDD-induced toxicity and malfunctions in the studied organs.· Green tea & curcumin administration reduced the amount of collagenous fibers in the studied organs. · Green tea & curcumin administration restored the amount of protein in the hepatic and renal tissues. · Green tea & curcumin administration restored the amount of carbohydrate material in liver, kidney and spleen. · The combined dose of (GT+Cur.) were found to have strong protective effect in the prevention of TCDD-induced toxicity; it was found that they reduced the amount of collagenous fibers, restored the amount of protein in liver and kidney and also restored the amount of carbohydrate material in liver, kidney and spleen. · Comparison between the recovery of the mice after TCDD administration by lifting the animals without any treatment or by giving them Green tea & curcumin. The results showed that green tea & curcumin significantly enhanced the recovery rat of mice. · It was found that the green tea & the combined dose of (GT+Cur.) exhibited more protective effect than curcumin. · It could be concluded that the protective role of green tea and curcumin against TCDD toxicity is clear in experiment I (protective) and experiment II (recovery). However, the protective role of green tea and curcumin against toxicity of TCDD in experiment I was more potent and more powerfull than that of experiment II (recovery). |