الفهرس | Only 14 pages are availabe for public view |
Abstract Mammalian sperm DNA is at least six fold more condensed than the DNA in somatic cells to protect the genetic integrity of sperm during its passage through male and female reproductive tracts. Thus a completely different type of DNA packaging must be present in mammalian sperm nuclei. · Human spermatozoa in which the chromatin is not completely condensed failed to fertilize, even after their injection directly into the ovum. So, it is of great importance to evaluate the chromatin status when testing the fertilizing capacity of spermatozoa. · The majority of laboratory tests can’t assess the man’s total fertility as many of these tests suffer from intra-observer variations and low numbers of spermatozoa analyzed. · These difficulties nowadays are approached through the development of computer-assisted semen analysis (CASA) that analyzes motion, morphometry and concentration. Furthermore, flow cytometry is a simple rapid procedure that quantities DNA content and chromatin condensation for all cells present in human semen. · The present study was aimed to compare between image cytometry, computer-assisted semen analysis (CASA) and flow cytometry (FCM). These methods are used to identify different spermatogenic cells. They can evaluate the chromatin structure in the ejaculated semen. This may be of value to predict the outcome of assisted reproductive techniques (ART) as intra uterine insemination (IUI), invitro fertilization (IVF) and intra cytoplasmic sperm injection (ICSI). · The study involved 60 cases classified into three groups: 10 normally fertile men who were used as a control group, 41 infertile men with abnormal spermatozoa ranging from 40-60 % and 9 infertile men with abnormal spermatozoa more than 60 %. · The significant parameters in the results of the infertility groups were observed in head area, head perimeter, ejaculate volume, sperm motility, linear sperm velocity, abnormal morphology percentage and chromatin condensation percentage. 118 · Flow cytometric analysis revealed significant differences concerning chromatin condensation and decondensation percentage in the sperms of the infertile groups. · Determination of chromatin condensation and decondensation percentage in the nuclei of spermatozoa obtained from infertile men is very important in the process of assissment of infertile capacity. · The use of a specific histology stain as Propedium Iodide could be considered as a new simple method of staining to evaluate the integrity of chromatin of the sperm nuclei. · The presence of spermatogenic cells in semen could be used to give an idea about how the testicle is currently functioning and predict the outcome of testicular sperm extraction (TESE) with oligo-spermia. So the use of image cytometry, CASA and flow cytometry for this task could be of help. |