الفهرس | Only 14 pages are availabe for public view |
Abstract C-kit positive cells outside the gastrointestinal tract (GIT) which are morphologically similar to interstitial cells of cajal (ICC) in the GIT were supposed to play same role of motility in organs other than the GIT. Aim of the work: To observe the effect of ischemia reperfusion injury I/R injury on the rat vas deferens with reference to interstitial cajal like cells (ICLC). Materials and Methods: This study was performed on 35 male albino rats weighing 180-200 gm divided into 3 main groups; control group (A), ischemia group (B) and reperfusion group (C) after applying spermatic cord torsion for one hour followed by detorsion. All sections from the different groups were stained with heamatoxylin and eosin (H & E), toludine blue and c-kit immunohistochemichal staining and morphometric study was done recording the mean area percent of c-kit positive (ICLC). Results: The examined H & E sections showed histological changes in ischemia group and the first two subgroups of reperfusion C1, C2 and relative recovery in the other two subgroups C3, C4. The mean area percentage of c-kit positive ICLC in the ischemia group and the first two subgroups C1, C2 of reperfusion was significantly decreased compared to the control. The other two subgroups C3, C4 of the reperfusion group showed relatively increasing of the mean area percentage of the c-kit positive ICLC compared to the control. Conclusions: it was concluded that I/R injury had evident histopathologicl effects on the vas deferens that affected also its ICLC. This changes might explain abnormal vas deferens motility in I/R injury and might explain some of infertility causes. |