الفهرس | Only 14 pages are availabe for public view |
Abstract Forty six random samples of cow milk were collected from different localities at Sharkia Governorate, Egypt in a sterile bottle and transferred to the laboratory in ice-box to maintain its chemical and microbiological status unchangeable. Each sample was mixed thoroughly before being divided into two parts. The first was examined for incidence of mastitis by applying CMT; WST; MSCC and chloride tests, the second was used for bacteriological examination (Isolation and identification of yersinia spp as well as Detection of the gene (virF) in Yersinia enterocolitica using PCR method). The results of CMT revealed that 13 (28.3%) of the examined cow’s milk samples were negative and 33 (71.7%) were positive. While, results of WST showed that 12 (26.1 %) of examined cow milk samples were negative and 34 (73.9%) were positive samples. The MSCC/ml. was studied and reveals that negative samples contained less than 3.55 x l 05 MSCC/ml., while the positive ones contained higher numbers of MSCC exceeded 6.8 x 106 /ml. There was a marked increase in SCC in cow udder affected with subclinical mastitis than that in normal milk. The mean value of chloride%/g of normal cow milk samples (CMT negative samples) was 0.105±0.027, while the CMT positive samples were 0.168±0.002. The comparisons by 2-tailed test compare the mean values for MSCC/ml and chlorides% in normal and mastitis milk samples showed significant difference of mean at 0.01 levels. |