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Abstract Cancer is a term for diseases in which abnormal cells grow and divide without control. This is usually the result of damage to a number of regulatory mechanisms within the cell (Cooper, 1992). These damaged cells grow to form a tumor - an abnormal mass of tissue, Cancer is a genetic disease (Vogelstein and Kinzler, 2002). Although environmental and other non-genetic factors such as cigarette smoking, exposure to ultraviolet light, diet and carcinogenic chemicals have roles in many stages of tumor genesis. In cancer, free radicals are generated in excess leading to damage of lipids, which leads to LPO (Senthil and Manoharan, 2004), lowered concentrations of glutathione (GSH), vitamin E and catalase (CAT) (Kolanjiappan et al., 2002) . There has been a great deal of interest recently in the role of complementary and alternative medicines for the treatment of various acute and chronic diseases (Seef et al., 2001). Bee venom is found to inhibit significantly nonenzymatic lipid peroxidation that antioxidant activity is involved in the anti-inflammatory activity of honey bee venom (Rekka et al., 1990). In the present study, Forty female mice weighing about 20-25 g were purchased from National Research Center, EL- Doky, Cairo, Egypt and maintained at the animal house of Genetic Engineering and Biotechnology Institute, Minufiya University. Mice were housed at 23 ± 2oC and in daily dark/light cycle. They were maintained under standard condition and fed standard chow and water ad libitum. Animals were classified into five main groups of eight animals each, as follows: -Group I: The Animals of this group served as normal controls and given only standard pellet diet and tap water. - Group II: Animals of this group were given standard pellet diet, tap water and were injected subcutaneously into the left footpad by 0.3 mg of bee venom / mouse for seven days. Mice were sacrificed 2 weeks post-injection. - Group III: Animals of this group were given standard pellet diet, tap water and were implanted with EAC to form a solid tumor. Mice were sacrificed 3 weeks post-injection. - Group IV: Animals of this group were given standard pellet diet, tap water and were injected subcutaneously (S.C.) into the left footpad by 0.3 mg of bee venom / mouse for seven days After 24h mice were implanted with EAC to form a solid tumor. Mice were sacrificed 2 weeks post-injection. - Group V: Animals of this group were given standard pellet diet, tap water and were implanted with EAC to form a solid tumor. After 2weeks mice were injected by bee venom subcutaneously (S.C.) in a volume of 0.3 mg / mouse for seven days. Mice were sacrificed 24h post-injection. |