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Abstract ick colonies were initiated from field ticks (El-Kharga Oasis, New Valley Governorate) where tick infestation is predominant. Adult whole tick extract and whole tick larval extract antigens were prepared from Hyalomma anatolicum anatolicum laboratory reared tick colony. Four groups of laboratory animals were selected in order to test the prepared antigens. The first and second groups were subdivided into group IA and IB, group IIA and IIB. The first group (IA and IB) was vaccinated with the adult WTE antigen while the second group (IIA and IIB) vaccinated with WLE antigen. After 28 days post the 1st dose of vaccination, all groups were challenged with laboratory reared ticks as follows, the first group IA was infested by 200 adult Hyalonnna anatolicum anatolicum tick per animal while group IB was infested by 200 larvae of Hyaloninia anatolicum anatolicum. The second group IIA was infested by 200 adult ticks whereas group JIB was infested by 200 larvae. Group III was infested by 200 adult tick per animal, the fourth group was infested by 200 larvae. Vaccinated groups responded normally to vaccination. The level of serum antibodies against tick antigen were high and continued while in control groups no levels of antibodies at this period. No clinical adverse reactions could be detected in vaccinated animals and heir body temperatures were normal as those of control. The used antigens proved to be safe as no detection of parasitaemia and schizont in lymph nodes of vaccinated animals throughout 4 weeks post vaccination was de ected. Vaccinated animals could withstand challenge with infest of laboratory reared ticks as the number of partially fed and reje ted ticks were very high than that of control groups. Whereas the number of full engorged females were lower than resulted from control oup. In addition, there were abnormal ticks resulted from the vaccinated group with WTE. On the contrary, no tick abnormalities resulted froi control groups. On studying the effect of Theileria annulata infected fi Id ticks on both vaccinated and control groups. It was resulted that the control groups suffered from severe infection than vaccinated groups. It could be concluded that the prepared tick antige s have potential for inducing immunity in calves. Moreover, adult antigen seems to be superior to the larval antigen. In addition, immu ization against ticks with antigens comprising tick-extract (larval an adult) could afford better protection against Hyalonnna anatollann ana’ olicurn tick infestation. In the meanwhile, this result does not lo er the necessary to control Theileria infection which carried by this ticks. |