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Abstract The present work was carried out on 168 adult albino rats (60-80 day old age). After acclimatization for 2 weeks, the rats were randomly classified into 7 groups, each group contain 24 rats as following: 1. Group I Control group (-ve control): served as control without any treatment for 8 weeks. These rats received normal food and water during the period of the study. 2. Group II (+ve control): served as experimental control and received ammonium metavanadate at concentration of 1/10 of LD50 about 5.62 mg/kg dissolved in 0.2 ml distilled water orally intragastric daily for 8 weeks. 3. Group III: Animals received ammonium metavanadate as in group II and were taken individual treatment of tiron (314 mg/kg/day dissolved in 0.2 ml distilled water) orally intragastric 4 days /week. 4. Group IV: Animals received ammonium metavanadate as in group II and were administered selenium (0.5 mg/kg dissolved in 0.2 ml distilled water) orally 4 days /week. 5. Group V: Animals received ammonium metavanadate as in group II and were administered vitamin E (50 mg/kg dissolved in 0.2 ml distilled water) orally 4 days /week. 6. Group VI: Animals received ammonium metavanadate as in group II and were administered tiron (314 mg/kg/day) and selenium (0.5 mg/kg, orally) 4 days /week. 7. Group VII Animals received ammonium metavanadate as in group II and were administered tiron (314 mg/kg/day) and vitamin E (50 mg/kg, orally) 4 days /week. Rats were observed daily. The body weight was recorded on the first day before injection (initial weight) and the day of sacrifice (final weight) every 2 weeks until 8th week. Blood samples were collected from which serum was isolated for various blood biochemical assays (malondiadehyde (MDA), superoxide dismutase activity (SOD) and testosterone assay). Immediately after blood collection, rats were rapidly sacrificed and tissue specimens from the liver and kidney were fixed in 10% neutral buffered formalin solution, while other organs testes and epididymis were fixed in Bouin’s solution for light histopathological examination. Small blocks (1m x 1m) from the liver tissues were additionally processed for transmission electron microscopy. The results of the present study showed that all rats in the control group were in good healthy condition and showed normal appearance, higher activity throughout the period of experiment. Rats of group II +ve control (ammonium metavanadate treated rats) had diarrhea, reduction in food consumption, loss of appetite and weight, loss of viability, depression toward the end of the experiment. No distinctive differences were observed in physical appearance and in motor behavior in all rats exposed to ammonium metavanadate plus chelation therapy (tiron) and/or antioxidants (vitamin E, selenium) compared to the controls during the whole period of the experiment. The post mortem examination of ammonium metavanadate-treated rats revealed marked emaciation and dehydration, the intestine was small, free from food with sever congestion and contain serous exudate and gases. |