الفهرس 
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Abstract
The thesis comprises four main parts:
Part I
This part presents an introduction about the chemical name, structures, physical properties
and pharmacological action of studied drugs. It also contains literature review for the reported
methods of analysis of the selected drugs in pharmaceutical dosage forms as well as in biological
fluids.
Part II
This part discusses the development and validation of an HPLC method for the
determination of Cefotaxime. A kinetic investigation of the drug in different stress conditions
was done. Also, it discusses the application of chemometrics to the handling of chromatographic
response data. This was done following the kinetic degradation of CEF under three stress
conditions: alkaline hydrolysis at three different temperatures 30, 50 and 70 ºC and acidic
hydrolysis using two different concentrations of HCl (0.05 and 0.1 M) at four different
temperatures 40, 60, 70 and 80 ºC. Derivative treatment (First and second) of chromatographic
response data of CEF at the previously mentioned eleven cases followed by convolution of the
resulting derivative curves using 8-points sin xi polynomials (Discrete Fourier functions) was
done. This was found beneficial in improving linearity parameters (r, a, b, sy/x and F) of the
response data. The application of Theil´s method, a non-parametric regression method, in
handling the response data was compared with the least squares parametric regression method,
which is considered the standard method used for regression. Theil´s method was found to be
superior to the method of least squares in chromatographic response data. Finally, Arrhenius
equation was applied on chromatographic response data after chemometric treatment as
mentioned above for the parametric and non-parametric treatment of data in order to determine
the half life (t1/2) of CEF in alkaline and acidic conditions at room temperature with high
accuracy and it was found that both parametric and non-parametric treatment led to the same
results. The estimated values of K25ºC and t1/2 at 25° C after the application of D2/FF method,
which is assumed to correct for all types of errors, were in a good agreement with the reported
values in literature.