الفهرس | Only 14 pages are availabe for public view |
Abstract In this study, prevalence of Cryptosporidium species among animals at Sharkia Province was investigated. Out of 150 examined animals (50 cattle, 50 sheep and 50 goats), 41 (27.33%) were positive by faecal examination. The incidence of cryptosporidioisis was 40%, 24% and 18% in examined cattle, sheep and goats, respectively. Calves were more infected than other animals. For characterization of the structure and transmission of natural and laboratory-propagated isolates, a collection of archived human and animal isolates of C. hominis, C. parvum and C. meleagridis were analyzed by deepsequencing polymerase chain reaction products amplified from a polymorphic sequence on chromosome 1. Thousands of screened 200-nucleotide sequences were analyzed to compare the diversity among samples, to assess the impact of laboratory propagation on population complexity and to identify taxonomically mixed isolates. Contrary to our expectation, repeated propagation in animals did not reduce intra-isolate diversity nor was diversity associated with host species. Significantly, in most samples, sequences characteristic of a different species were identified. The presence of C. hominis alleles in C. parvum and C. meleagridis isolates confirms earlier reports of mixed isolates and raises the possibility that the host range of C. hominis is broader than typically assumed. In a genetically divergent isolate of C. parvum, a majority of sequences was found to be recombinant, suggesting that this genotype originated from a C. parvum × C. hominis recombination event. GP60 sequences analysis of Thailand samples of six HIV-human cases and four calves faecal samples infected with cryptosporidiosis, showed that |