الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was conducted to develop molecular genetic fingerprints for identification and discrimination of 45 Date palm cultivars grown in Egypt, especially Ismailia Govemorate. Through, the detection specific molecular markers or (unique bands) of the Date palm cultivars are used, also, to estimate and elucidate the genetic relationships among these cultivars. In addition, detection of specific molecular markers seemed to be linked to some economically important characteristics in Date palm, i.e. fruit size, fruit color, Moisture content, time of Marketing and Average production by tree. Simple Sequence Repeats (SSRs) marker was used to perform these aims. For SSR markers the total number of amplified bands was 1067 bands (alleles) detected by 33 SSR loci and the average number of affected alleles was 12.82, all bands were (scorable polymorphic markers) polymorphic represented a level of polymorphism of lOO %. Some of SSR loci «DPALM_lOO, DP 151, and mpdCIR032))) were more successful in identifying genotypes than the others where they generated higher number of unique bands. This represented a level of polymorphism among the studied genotypes. The similarity coefficient reached a highest value of (0.259), between ”Bent-ashaa” and ”Hayani’ / Al-raghy and reached a minimum value of (0.041) between ”khalas’ and ”Hayani’? El Kasasen Results of the present study revealed, some SSR specificmarkers, which seemed to be linked to the inheritance of some economically important characters in Date palm cultivars. Key words: Date palm ( phoenix dactylifera L.) - Genetic fingerprints - Cultivar identification - diversity - cluster analysis - SSR - Horticultural characteristics.. |