الفهرس 
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Abstract
A.baumannii is an important pathogen during the last few decades in hospitals which is resistant to the action of many antibiotics and spreads easily from patient to another and causes extended epidemic outbreaks. Carbapenems were the most antibiotics in treatment of A.baumannii infections. The blaOXA genes were detected in A.baumannii strains causes MBLs enzymes production that made A.baumannii multi-drug resistant bacteria to many antibiotics.Samples were isolated from blood, urine, sputum and wounds of patients. The isolated A.baumannii strains (44 strains) were tested for antibiotic sensitivities included carbapenems. We found 32 isolates (73%) were imipenem sensitive, while 29 from the isolates (66%) were meropenem sensitive and 22 isolates (50%) were sensitive to amikacin.MBL production in carbapenems resistant A.baumannii was detected by the modified Hodge test where 54.55% were positive Hodge test while 45.45% were negative. The blocks genes were detected by PCR, showed 72.72% carried blaOXA-23 gene and 29.54% carried blaOXA-58 gene. A.baumannii sensitive or resistant to antibiotics were intraperitoneal injected infected mice, where after 3 days we found Statistically significantly increase of liver functions (ALT & AST) in serum of infected mice (P < 0.001) in both group compared to control group, while total bilirubin is significant increase in resistant group only and statistically significantly decrease of Glutathione (GSH) (P < 0.0001) and in activities of superoxide dismutase (SOD) (P<0.0001) and catalase (CAT) (P< 0.0001) in serum of infected mice compared to control group. While there was non-significant difference obtained in kidney functions markers between both infected mice groups compared to control group. Based on the above results, we can conclude that: •Carbapenem resistance in ICUs may be due to MBLs enzymes. Imipenem is more effective than meropenem in treating A.baumannii infections.•The Modified Hodge test is the main, easy, simple and specific test which used in the detection of MBL production.•The treatment of A.baumannii infections by using carbapenems is considered the most effective means to control the spreading of A.baumannii isolates in ICUs, to keep people safety and healthy in hospitals. •Resistant A.baumannii causes decline in liver functions and depleted estimated antioxidants in mice, while its effect is very limited on kidney functions that reflect the fast and sensitive effect on liver.