الفهرس 
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Abstract
Summary & Conclusion
Our study included total 72 subjects 29 apparently normal healthy (not receiving anticoagulant) as control (group A) and 43 receiving anticoagulant (17 on warfarin and 26 on heparin) (group B), both groups were matched age and sex.
Each sample of each group was analyzed for aPTT, PT, D-dimer and Fibrinogen immediately and after 4hrs, 8hrs, and 24hrs of storage. Also each sample was tested at room temperature (18-22 °C) and at refrigerator temperature (4 °C).
In coagulation studies, the diagnosis of coagulation disorders and monitoring of anticoagulant therapy usually depend on PT and aPTT values.
In general, coagulation tests should be carried out as soon as possible after collection of the blood samples as was demonstrated that after as little as 1 h, samples stored at RT may showed significant differences compared with baseline results, although these differences would not have a significant impact on clinical diagnosis or treatment decisions. Thus, a statistically significant difference need not be the same as a clinically relevant difference.
To conclude, we found that samples for PT were acceptable for analyses up to 24 h in those not receiving anticoagulant and up to 8 h in those receiving anticoagulant, aPTT samples can be tested up to 8 h in those not receiving anticoagulant and up to 4 h in those receiving anticoagulant whether stored at room or refrigerator while fibrinogen samples can be analyzed up to 4 h in both groups. However D-dimer samples should be analyzed immediately in those receiving anticoagulant and up to 24 h in those not receiving anticoagulant whether stored at room or refrigerator.
Recommendations
- We recommend that PT samples not to be stored more than 24 h whether in 25°C or 4°C temperature in subjects’ not receiving anticoagulant and up to 8 h in patients receiving anticoagulant.
- We recommend that aPTT samples not to be stored more than 8 h whether in 25°C or 4°C temperature in subjects’ not receiving anticoagulant and up to 4 h in patients receiving anticoagulant.
- We recommend that Fibrinogen samples not to be stored more than 4 h whether in 25°C or 4°C temperature.
- We recommend that D-dimer samples to be tested immediately in those receiving anticoagulants and up to 24 h in subjects’ not receiving anticoagulant.
- We recommend that more studies with larger numbers must be done to find the exact relation between storage time and changes in results of coagulation profile.
- We recommend that more studies with larger numbers must be done to find the exact relation between different temperatures and changes in results of coagulation profile.