الفهرس | Only 14 pages are availabe for public view |
Abstract 1- A total of 200 cases of diseased and recently dead different poultry species (100 broiler chickens, 50 laying hens, 30ducks and 20 turkeys) were collected from different localities in El- Ismailia Governorate. E. coli was isolated from 102(51%) cases. Only 10 E. coli isolates were serotyped as O111:K58, O1:K1andO146:K99 in order of frequency (60%, 20%and 20%) isolates, respectively selected 13 E. coli isolates were tested for their susceptibility to 13 antimicrobia agents and absolute resistance was obtained among selected E. coli isolates against Amoxicillin clavulanic acid (100%), Chloramphenicol (76.9%) and Erythromycin (76.9%). In addition, (69.2%) of isolates were resistant to Nalidixic acid, Rifamycin, Streptomycin and Cefoxitin and (61.5%) of isolates were resistant to Ceftriaxone and all tested isolates were resistant to at least 4 antibiotics and multidrug resistance was seen. The highest sensitivity rates were recorded to Ciprofloxacin (84.6%) and Colistin sulphate (76.9%). PCR results indicated that representative 10 E .coli isolates had antibiotic resistance genes a dfrA1, aada1, blaTEM and Sul1 genes 100 %( 10/10), while only 40 % (4/10) had floR gene. |