الفهرس 
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Abstract
In the present study, 1000 samples (liver, spleen, lung and trachea) from apparently healthy and diseased broiler, layer and baby chicks and 200 human samples bronchoalveolar lavage BAL, blood stream infection BSI, urine UR, wound W tissue T were collected to investigate the prevalence of Klebsiella spp. The isolation of Klebsiella from broilers revealed 4 isolates (5.8%), 8 isolates(5.3%) from layers, 3isolates from baby chicks (9.6%) and 16 human isolates (7.5%).Antimicrobial sensitivity testing against16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, Cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole/trimethoprim. Serological survey using; Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 6 isolates were positive for blaTEM gene and 5were positive for blaCTX gene. All isolates were negative for blaSHV gene.