الفهرس 
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Abstract
Thirty seed samples of soybean from different growing fields in different locations of Dakahlia and Damietta governorates were collected and used in this investigation. A total number of 31 species belonging to19 genera of fungi were observed. There were differences observed between the SB (17 genera and 29 species) and AP (18 genera and 28 species). Macrophomina phaseolina was found (up to 27.3%) and it was used as test pathogen for further studies. Seven Trichoderma isolates recovered from the previous seed health test were tested as bioagents against M. phaseolina. The cell-free extracts of the seven Trichoderma isolates were tested against M. phaseolina by agar well diffusion, T44sn isolate was the best antagonistic isolate against the pathogen, which recorded 2.47 cm inhibition zone of the pathogen growth, followed by T42sn 2.20 cm. The selected fungal strain (Trichoderma sp. T44sn) was identified using the molecular technique (18S rRNA) as T. viride. The present Trichoderma isolate was found to be a reasonable producer of chitinase (0.20 U). The chemical composition of the metabolic extract of T. viride T44sn was analyzed using Gas chromatography-mass spectrometry. Twenty-five compounds were identified in the culture filtrate of T. viride. A formula of T. viride was prepared, its shelf-life was investigated. Arbuscular mycorrhiza and formulated T. viride were applied individually or combined under greenhouse and field conditions. Finally, the present investigation recommended using a mixture if the proposed formulated T. viride combined with AM for the complete management of soybean diseases and increase its productivity.