الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Foot and mouth disease (FMD) is a highly infectious viral disease which affects all cloven hoofed animals. It is distributed all over the world and characterized by fever, foamy salivation, vesicles and erosions on the tongue, the teets, coronary band and inter-digital space. Sever inflammation may lead to separation between hoof and digit that lead to lameness and inability of animal to walk.FMD is an airborne worldwide disease caused by single stranded RNA viruses related to genus Aphtovirus of the family picornaviridae. It is assoicated with severe economic losses due to loss body weight of infected animal, decrease milk production, mortality in young animals, banning of exporting of animal product and byproduct, and vaccination costs of various serotypes of FMDV.The current study was performed on 25 serum and plasma samples collected from 15 FMD infected cattle and 10 apparently healthy cattle from farms in Dakahlia governorate, Egypt for estimating biochemical parameters as BHBA, NEFA, glucose, insulin, lipase and amylase. Moreover, 12 tongue epithelium (10 from infected animals and 2 from non- infected animals for Real time PCR and RT-RPA assays.The result reveled that:1.Increased BHBA and NEFA levels in FMD infected cattle that indicate ketosis.2.Decreased insulin levels in FMD-infected than control animals.3.Glucose levels were higher in infected animals than controls.4.Lipase activity was higher in infected animals than controls.5. Decreased amylase activity in FMD-infected animals.6.RT-PCR positive samples when tested by RT-RPA only 70% gave positive while 30% gave false negative.7.RT-PCR negative samples for healthy animals when tested by RT-RPA gave 100% negative result.These results suggest that FMD is accompanied by ketosis and possibly a pancreatic damage as shown by decreased insulin concentration and hyperglycemia.RT-PCR is more accurate than RT-RPA. However, RT-RPA is more quick and do not require specialized technician, performed at room temperature. Therefore, it can be used for rapid screening of virus in ports but negative samples should be re-tested by RT-PCR.