الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was carried out at Poultry Production Research Farm, Poultry Research Unit, Biological Application Department, Nuclear Research Center, Egyptian Atomic Energy Authority, during the period from Marsh to May 2017, and the chemical analysis were conducted in the Biological Applications Department laboratories, Nuclear Research Center, Egyptian Atomic Energy Authority. A total of 210 broiler breeder eggs (Habbard Star-Bro) were obtained from a local hatchery (El-Tokhy Co., New Salhia, Egypt) from a maternal flock 54 week age. Eggs were incubated at 37.8ᵒC and 60% RH. Upon arrival, the eggs were individually weighed and divided into three in ovo treatment groups. The first treatment non in ovo-injected, and considered the control group, while the second and the third treatments were in ovo injected with 100 μL of nano –Selenium(elemental selenium ) with concentrations of 5, 10 ppm respectively at 14 day of incubation. The hatched chicks, from each treatment were randomly divided into two treatments , once was added 10 PPm nano -Se per kilogram ration, and the other without any additive, it had became six treatments . Data were collected in terms of live body weight, weight gain, feed consumption and feed conversion during the experimental period. At the end of the experiment, three birds from each group were slaughtered, eviscerated to determine carcass characteristics and blood samples were collected. At autopsy, representative tissue samples were taken from pectoral muscle, ileum, liver, cecal tonsils, spleen, thymus and bursa of Fabricius for histological examination. The results obtained can be summarized as follows: 1.Hatchability rates were reduced significantly as influenced by different experimental treatments while body weight at hatch did not affected.interaction between their, however, Cholesterols and LDL were not affected by the treatments. 11. Serum reduced glutathione content and glutathione reductase activity were increased significantly as affected by the two nano- Se delivery routes and the interaction between there. 12. Malondialdehyde content was decreased significantly as affected by the two nano-Se delivery routes and the interaction between there. 13. Serum IgM, IgG, IgA, total Ig and antibody titer against NDV were increased significantly as affected by feed supplementation nano- Se and in ovo injection nano-Se, while the interaction between the two nano-Se supplementation routs did not affected on the previous parameters. from the previous results it could be concluded that: In ovo inoculation selenium nanoparticles at14th day of embryogenesis or supplementation it as feed additive or both the two routes together could be used as a good tool for improving subsequent post hatch productive performance,lipid profile, antioxidant and Immunological status of broiler chicks. Based on the present results, it is recommended to use selenium nanoparticles especially with In ovo doses of 10 or 5ppm per egg with 10ppm per kilogram ration respectively, in order to improve productive, physiological and immunological status of hatched chicks. |