الفهرس | Only 14 pages are availabe for public view |
Abstract The objective of this study was to assess the hygienic quality and safety of rice milk and mahallbia sold in Mansoura City, Egypt, through detection of the prevalence of B. cereus in some locally manufactured dairy desserts that sold in Mansoura city, Moreover, the enterotoxigenicity of recovered isolates was detected through scanning the isolates for some virulence genes.. Rice milk and mahallbia are very popular dairy desserts consumed in Egypt that are processed and stored in conditions suitable for many microorganisms to flourish. Bacillus cereus is a Gram-positive, spore-forming pathogen that was incriminated in several foodborne outbreaks of two distinct types; the diarrheal and the emetic. Both types have serious effect on human health. In this study, One hundred samples of dairy desserts (Mahallbia and Rice milk, 50 of each) were examined for detection, enumeration and molecular examination of B. cereus. B. cereus could be detected in 58 , 74% of mahallbia and rice milk samples with mean values of 8.38× 105±1.8×105 and 4.4 × 106 ± 1.4 × 106CFU/g, respectively. It was found that 100, 77.8, 100 and 22.2 % of B. cereus isolates obtained from the examined samples have nhe, hbl, cytK and ces genes, receptively. It could be concluded that other bacillus species were isolated as showed; Bacillus subtilis in 70% from examined mahallbia samples, 84 % rice milk samples. B. pumilus could be detected in 30% and 58% from mahallbia and rice milk samples; respectively. B. lichenformis presented in 18% and 24% from mahallbia and rice milk samples, respectively. |