الفهرس 
Aim of the workOnly 14 pages are availabe for public view
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Abstract
Bladder cancer ranks eighth among newly diagnosed cases worldwide, we found that searching for modern methods that avoid long complications and costly tests that depend on building a microbial fingerprint, especially bacterial bladder cancer, will facilitate the diagnosis process and thus lead to faster treatment. There is a close relationship between one or more specific bacterial types, with bladder cancer tissues, in this study, using conventional microbiological techniques, 20 bacterial colonies that were collected from the Urology and Nephrology of Center - Mansoura University, were isolated. Molecular biological techniques such as DNA fingerprinting have also been used, and patterns of protein range SDS-PAGE ٬and 16S rRNA gene sequence and MALDI-TOF-MS and plasmids ٬extraction, where three types were found, including E. coli, one P. aeruginosa, and one Klebsiella pneumonia. The study demonstrated that the MALDI-TOF-MS scale is highly accurate in defining Bacteria are the same as determining the nucleotide sequence of a 16S rRNA gene with a significant difference in speed, accuracy, and costs• Detection of pathogenic bacteria in the tissues of bladder cancer. The use of classical methods, compared with modern methods used to detect pathogenic bacteria Build a bacterial fingerprint in human patients with bladder cancer who have been positively diagnosed with bladder cancer In order to achieve these goals, 10 tissue samples from bladder cancer patients were collected from the Urology and Nephrology of Center - Mansoura University, where it was cultivated and purified in the bacteriological laboratory of the Faculty of Science, Mansoura University, where it underwent the following tests. A sample was taken from the tissue of the bladder cancer and cultivated it on LB agar plates, after incubation at 37 ° C for 48 hours, where pure colonies were obtained, after which they were subjected to Gram stain. Antibiotic sensitivity / resistance testing was performed for all isolated bacterial specimens, using tablets saturated with the following antibiotics: Vancomycin (30 µg), Penicillin (10µg), Kanamycin (30 µg), Trimethoprim / Sulphamethoxazole (25 µg), Gentamycin (10 µg), Ampicillin (10 µg), Erythromycin (15 µg). Through this test it was found that the isolates numbers A3, B3, A5, B5, A9 are resistant to many antibiotics. A protein analysis of antibiotic-resistant isolates was performed, as B3, A5 and B5 isolates showed similarities in protein patterns, whereas A9 and A3 isolates showed differences with all patterns. Bacteria was identified and defined by modern identification methods including protein fingerprint, genetic fingerprint, genetic fingerprint 16S rRNA, and definition of MALDI-TOF-MS Key words: Antibiotics, Molecular Protein Analysis, Genetic Footprint, MALDI-TOF-MS Definition, 16S rRNA Gene Sequence Analysis.