الفهرس | Only 14 pages are availabe for public view |
Abstract Marine yeasts have several promising features for biotechnological applications. In this work, a total of 120 yeast isolates were recovered from marine sediments and seaweeds of five different locations along Suez Canal and Suez Gulf. The isolated yeasts were grouped into nine yeast groups according to their phenotypic and genotypic identification. The biodiversity and distribution patterns analysis of the isolated yeasts were conducted. Screening of glycerol production potentiality indicated that all the tested isolates could produce glycerol at variable concentrations.The influence of (UV) irradiation on glycerol production revealed that; among the tested isolates, Wickerhamomyces anomalus HH16 MU5 showed the highest glycerol production (80.15 g/l). Optimization of the fermentation parameters by the mutant W. anomalus HH16 MU5 was achieved. The maximum glycerol production 86.55 g/l has been attained under aerobic conditions in a medium composed of 200 gl-1 glucose, 1 gl-1 peptone, 3 gl-1 yeast extract, and 58.44 gl-1 NaCl, this medium was adjusted at pH 8 and incubated for 3 days at 30 oC. Results also indicated the feasible production of glycerol using agro-industrial wastes (fruit peels and sugarcane bagasse), however the waste type and the pretreatment process mainly affect on the glycerol yield and the cellulase activity. |