الفهرس | Only 14 pages are availabe for public view |
Abstract Utilization of some agro industry wastes in Production of fungal lipases. Fungi are used in several industrial processes, such as production of enzymes. Importance of enzymes is increasing especially microbial lipases which are of indefinite industrial purposes. Solid state fermentation is an economical alternative for large scale production of enzymes which produced by fungi. By using solid state fermentation, the production of extra- cellular lipase from filamentous fungi isolated from soil and the oil contaminated soil using agricultural wastes has been studied. Seven fungal strains were isolated from twenty soil samples and then tested for lipase production. Among these fungi, Aspergillus flavipes was identified and selected as highest lipase producer using Nigella sativa as a waste among seven agricultural wastes. The optimum conditions for lipase production were pH 7.2, temperature 28°c and 6 day incubation period. Maltose and yeast extract were the best carbon and nitrogen source respectively. The Plackett- Burman statistical design was applied; The Box-Behnken design was carried out according to the results of the main effect obtained from Plackett-Burman. One of the applications of lipase is biodiesel production from waste vegetable oil, biodiesel production using lipase extracted from A. flavipes yield 29.5 mg/l biodiesel products. |