الفهرس | Only 14 pages are availabe for public view |
Abstract Summary and Conclusion The main objective of pulp therapy in primary dentition is to maintain every tooth as a fully functional component in the dental arch to allow for proper mastication, phonation, swallowing, preservation of the space required for eruption of permanent teeth and prevention of harmful psychological effects due to tooth loss. The aim of this study was to evaluate the clinical and radiographic effect of chitosan and chitosan loaded antibiotics as a pulpotomy agent in non-vital primary molars also, assess the release of antibiotics after incorporation into chitosan formulation and assess their antibacterial effect. The study was conducted on sixty healthy children aged between four and seven years from the outpatient clinic of Pediatric Dentistry Department, Faculty of Dentistry, Tanta University. The sixty children had bilateral carious mandibular primary molars indicated for non vital pulpotomy; the 120 selected teeth were divided randomly into three groups according to the material used. The group I was treated with unprocessed chitosan powder in one side with the other side being treated with 3Mix-MP, while the group II was treated with unprocessed chitosan powder in one side with the other side being treated with chitosan loaded antibiotics. Finally the group III was treated with chitosan loaded antibiotics in one side with the other side being treated with 3Mix-MP. The cases were followed up clinically and radiographically for three, six, nine, twelve and 18 months. For in vitro drug release evaluation of the prepared formulae , the drugs were analyzed by ultraviolet spectrophotometry. For amoxicillin, stock solution was prepared in distilled water to produce 1mg/ml. The absorbance was recorded at 230 nm. For ciprofloxacin, stock solution was prepared in ethanol. The absorbance was recorded at 278nm. The same procedure was utilized to construct the calibration curve of metronidazole but the absorbance was recorded at 310nm. The results were plotted as a function of drug concentration to construct the standard calibration curve. The dissolution studies utilized the United States Pharmacopeia (USP) paddle method. The dissolution medium is 900 ml of 10 Mm phosphate buffer was maintained at 37+0.1oC and the paddle was rotated at 75 rpm, before loading (250mg and equivalent amount of the chitosan coated particle (. Samples of 5 ml were withdrawn at predetermined time. The samples were properly diluted. Each dissolution test was performed in triplicates with separate dissolution study being conducted for each drug. The cumulative amount dissolved was plotted as a function of time to produce the dissolution profile. For the bacteriological assessment, antimicrobial effect of chitosan, chitosan loaded three antibiotics and 3Mix-MP against S. aureus and E. coli was determined by agar diffusion method on Muller-Hinton agar and this was done in microbiology laboratory at Microbiology Department, Faculty of Medicine, Tanta University. Bacterial suspensions equivalent to 0.5 McFarland were freshly prepared from pure bacterial culture of the isolated organisms. The tested organism was spread on the surface of Muller Hinton agar plate. |