الفهرس 
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Abstract
This Thesis Consists Of Three Parts; The First Part Includes An Introduction And Literature Review For Each Of The Studied Drugs, The Second And The Third Parts Include Descriptive Experimental Work For The Studied Drugs. It Ends With References And A Summary In Arabic.
PART I: INTRODUCTION AND LITERATURE REVIEW
This Part Includes:
Section A: Introduction And Literature Review Of Mebendazole And Quinfamide
This Introduction Describes The Pharmacological Action Of Mebendazole And Quinfamide, Their Chemical Structure, Physical Properties And Review Of The Published Methods Developed For Their Analysis.
Section B: Introduction And Literature Review Of Dacarbazine
This Introduction Describes The Pharmacological Action Of Dacarbazine, Its Chemical Structure, Physical Properties And Review Of The Published Methods Developed For Its Analysis.
Section C: Introduction And Literature Review Of Felodipine And Metoprolol Succinate
This Introduction Describes The Pharmacological Action Of Felodipine And Metoprolol Succinate, Their Chemical Structure, Physical Properties And Review Of The Published Methods Developed For Their Analysis.
PART II: DIFFERENT chrOMATOGRAPHIC METHODS FOR DETERMINATION OF THE STUDIED DRUGS.
This Part Includes:
Section A: RP-HPLC And HPTLC For Simulataneous Determination Of Mebendazole And Quinfamide.
In This Section, Eco-Friendly RP-HPLC And HPTLC Methods Were Applied For Determination Of Mebendazole And Quinfamide. For RP-HPLC Method, Isocratic Separation Of Both Drugs Was Carried Out Using A Mobile Phase Consisting Of Double Distilled Water: Methanol (30: 70, V/V) At A Flow Rate Of 0.8 Ml Min-1 And UV Detection At 254 Nm. For HPTLC Method, Separation Was Obtained Using A Mobile Phase Consisting Of Methanol: Toluene (2:6, V/V) And The Separated Bands Were Scanned At 254 Nm. The Developed Methods Were Successfully Applied For Determination Of Mebendazole And Quinfamide In Their Pharmaceutical Formulation.
Section B: RP-HPLC And HPTLC For Simultaneous Determination Of Dacarbazine And Its Related Impurities
In This Section, Eco-Friendly RP-HPLC And HPTLC Methods Were Applied For Determination Of Dacarbazine And Its Related Impurities. For RP-HPLC Method, Isocratic Separation Was Performed Using Double Distilled Water: Methanol (70: 30, V/V) As A Mobile Phase With A Flow Rate Of 0.6 Ml Min-1. UV Detection Was Carried Out At 230 Nm. For HPTLC Method, Separation Was Performed Using Butanol: Ethyl Acetate: 0.05M Ammonium Acetate (2: 4: 0.4, By Volume) As A Green Developing System. Separated Bands Were Scanned Densitometrically At 280 Nm. The Developed Methods Were Successfully Applied For Determination Of Dacarbazine In Its Pharmaceutical Formulation.
Section C: RP-HPLC And HPTLC For Simultaneous Determination Of Felodipine, Metoprolol Succinate And Their Major Metabolites.
In This Section, RP-HPLC And HPTLC Methods Were Applied For Determination Of Felodipine, Metoprolol Succinate, And Their Major Metabolites. For RP-HPLC, Separation Of The Studied Components Was Obtained By Gradient Elution Using A Mobile Phase Composed Of Double Distilled Water (Adjusted To Ph 3.5 With O-Phosphoric Acid): Acetonitrile: Methanol (45: 40: 15, By Volume) For The First 6 Minutes And (30: 60: 10, By Volume) For The Next 4 Minutes At A Flow Rate Of 1 Ml Min-1 Followed By UV Detection Of The Eluted Peaks At 225 Nm. For HPTLC Method, Separation Was Achieved Using A Mobile Phase Consisting Of Toluene: Ethyl Acetate: Methanol: Ammonia: Formic Acid (10: 5: 2.5: 0.3: 0.1, By Volume) And Scanning Of The Separated Bands At 225 Nm. The Developed Methods Were Successfully Applied For Determination Of Felodipine And Metoprolol Succinate In Their Pharmaceutical Formulation And In Spiked Human Plasma.
PART III: DIFFERENT SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF THE STUDIED DRUGS.
This Part Includes:
Section A: Different Spectrophotometric Methods For Determination Of Mebendazole And Quinfamide.
In This Section, Four Eco-Friendly Spectrophotometric Methods Were Developed For Determination Of Mebendazole And Quinfamide. Mebendazole Was Determined By Extended Ratio Subtraction Method At 249 Nm (Λmax), While Quinfamide Was Determined By Iso Absorptive Point Method At 283.4 Nm (Λiso). Simultaneous Ratio Subtraction Was Applied For Determination Of Mebendazole At 249 Nm (Λmax) And Quinfamide Using Its Ratio Spectra At 273 Nm. Mebendazole Was Determined By Absorbance Subtraction Method At 283.4 Nm (Λiso) By Multiplying Its Absorption At 357.6 Nm By The Absorbance Factor Which Equals 2.2, While Quinfamide Was Determined By Subtracting The Absorbance Of Mebendazole At 283.4 Nm (Λiso). Dual Wavelength Method Was Also Applied For Determination Of Both Drugs Using The Absorbance Difference Of The Zero order Spectra Between 248.8 And 277.4 Nm For Mebendazole, And Between 266.2 And 310 Nm For Quinfamide. The Developed Methods Were Applied Successfully For Determination Of Mebendazole And Quinfamide In Their Pharmaceutical Formulation.
Section B: Different Spectrophotometric Methods For Determination Of Dacarbazine And Its Related Impurities.
In This Section, Innovative And Eco-Friendly Double Dual Wavelength, Dual Ratio Subtraction Spectrophotometric Methods Were Carried Out Along With Successive Ratio Subtraction Spectrophotometric Method For Determination Of Dacarbazine And Its Related Impurities. Double Dual Wavelength Was Applied For Determination Of Dacarbazine Using The Absorbance Differences Of Its Zero order Absorption Spectra Between 323 And 350 Nm. Dual Ratio Subtraction Method Was Applied For Determination Of 5-Amino-Imidazole-4-Carboxamide By Applying Ratio Subtraction Method Followed By Dual Wavelength Method Using The Absorbance Differences Between 267.2 And 286.2 Nm Of Its Zero order Absorption Spectra. Successive Ratio Subtraction Was Applied For Determination Of 2-Azahypoxanthine At 235 Nm. The Developed Method Was Successfully Applied For Determination Of Dacarbazine In Its Pharmaceutical Formulation.
Section C: Different Chemometric Methods For Determination Of Dacarbazine In Presence Of Its Related Impurities.
In This Section, A Comparison Was Carried Out Between Different CLS Based Chemometric Methods Which Are Classical Least Squares Regression (CLS), DCLS (CLS Coupled With First Derivative Data), OPLS-CLS (CLS Coupled With OPLS Preprocessed Data) And Spectral Residual Augmented Classical Least Squares (SRACLS). The Comparison Shows Models characteristics Through Their Application To Analyze Dazcarbazine (DTIC) In Presence Of Its Related Impurities. The Developed Methods Were Successfully Applied For Determination Of Dacarbazine In Its Pharmaceutical Formulation.
Section D: Different Spectrophotometric Methods For Determination Of Felodipine And Metoprolol Succinate In Presence Of Their Major Metabolites.
In This Section, Felodipine Was Determined Directly Using Its Zero order Spectra At 366 Nm. Triple Divisor Spectrophotometric Method Was Applied For Determination Of Metoprolol Succinate Using The Amplitude Values At 235.6 Nm Of The Second Derivative Of Ratio Spectra Of Metoprolol Succinate Resulted from Using A Mixture Containing Equal Concentration Of Each Of The Three Other Components As A Triple Divisor. The Developed Methods Were Successfully Applied For Determination Of Felodipine And Metoprolol Succinate In Their Pharmaceutical Formulation.
This Thesis Contains 109 References, 84 Figures, 44 Tables And Ends With An Arabic Summary.