الفهرس | Only 14 pages are availabe for public view |
Abstract In the past two decades, dental materials have been widely improved and along with the great development in tissue engineering and stem cell biology led to open new era toward dental tissue regeneration and differentiation. The aim of our study was to evaluate and compare the effect of mineral trioxide aggregate (MTA) and tri-calcium silicate cement (Biodentine) on proliferation and differentiation of dental pulp stem cell (In vitro study) and to evaluate and compare their effects on root regeneration in immature necrotic permanent teeth. (In vivo study). Pulp tissues of human vital impacted third molar teeth were removed under sterile conditions and populations of dental pulp cells were isolated using enzyme-digestion method, cultured and expanded in-vitro in culture growth medium. The cultured dental pulp stem cells were characterized by monitoring their growth, self-renewal capability, Multidifferentiation lineages and colony formation during the culture period. Also, the relative expression of stem cell genes (Bmi1 & Stat3) in the cultured cells was evaluated using quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR). Summary and Conclusions |