الفهرس | Only 14 pages are availabe for public view |
Abstract One of the important livestock economies are camels which adapt with adverse environmental conditions and provide milk, meat, wool, hides, and skin. Gram-negative Pseudomonas aeruginosa is harbor multidrug antimicrobial resistance of camel has serious consequences for human health, SO, this study aimed to characterized of P. aeruginosa especially extended spectrum β-lactamases (ESBL) producing ones; phenotypically and genotypically. 30 isolates of Pseudomonas aeruginosa with a percent of 12% from total of 250 nasal swabs apparently healthy (150) and diseased camels with respiratory manifestations (100) by cetrimide agar medium. The isolates were confirmed biochemically by GN card of Vitek 2 compact system (bioMe´rieux). Suspected ESBL P. aeruginosa colonies were 56.6% )17\30) by the double disc synergy test (DDST). Antibiotic sensitivity test showed that the P. aeruginosa were (100%) resistance to 3rd generation cefotaxime and 4th generation cefepime, followed by carbapenem: Meropenem and Imipenem (88.2%) and (82.3%), penicillin (82.3%), gentamicin (76.4%), aztreonam (70.5%), erythromycin (29.5%),sulphamethoxazole/trimethoprim (29.5%), and highly sensitive for ofloxacin (100% sensitive) Molecular Detection of virulence genes using pslA, toxA and exoU genes revealed that 29.4%, 23.5% and 17.6% were positive respectively. Detection of ESBLs encoding genes in P.aeruginosa recorded that blaTEM ,blaSHV and blaCTXM genes were detected in percentages of 64.7%, 47.0 % and 29.4%, respectively. Finally, ESBL P. aeruginosa showing multidrug antimicrobial resistance that detected by mexR gene. |