الفهرس 
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Abstract
Dietary components have recently received rapidly expanding attention for their potential to halt or reverse the development of many oxidative stress-mediated diseases after exposure to environmental toxicants. 7, 12 Dimethyl Benz(a)anthracene (DMBA) is one of the most common environmental pollutants. The aim of the present study was to evaluate the chemopreventive effects of Broccoli as nutritional components against DMBA intoxication in rats. A daily dose of Aqueous and methanolic broccoli extracts (1ml/Rat,150mg/kg) respectively were given to 50 days old female rats for 26 successive weeks after carcinogen intoxication with a single dose of (20mg/ml) of DMBA. DMBA intoxication resulted in redox imbalance (decreased GSH level and increased MDA level), and increased DNA damage fragmentation in liver, kidney and brain and bcl2 gene in kidney and brain. Besides, it affected the level of expression of GST gene in liver, kidney and brain tissue but not affected cfos gene level of expression. The aqueous and methanolic broccoli extract supplements ameliorated the adverse effects induced by DMBA intoxication by increasing the level of GSH and decreasing the MDA level with concomitant lowering in DNA fragmentation, decreased expression of GST and bcl2 in liver and brain, respectively as well as up-regulated GST and bcl2 expression in kidney. Broccoli as dietary components had a strong chemoprotective effect against oxidative stress, DNA damage and genotoxicity induced by DMBA intoxication in rats.