الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this study was to investigate the effect of sesame oil on proliferation and level of basic fibroblast growth factor expression of periodontal ligament fibroblasts, isolated from either healthy teeth or teeth diagnosed with chronic periodontitis. The current study included 8 health teeth free from inflammatory periodontal disease and indicated for extraction for orthodontic treatment and 8 hopeless teeth with chronic periodontitis indicated for extraction. Fibroblasts were isolated from teeth periodontal ligaments and flowcytometry was used to confirm their identity. Cultured cells were divided into two groups, sesame oil group was treated by sesame oil in a dose 2.5 μl of oil/ml of media at 24 hours and untreated group which served as control. Microtiter plate assay was used to assess the proliferation of both control and sesame oil treated cells while quantitative Polymerase chain reaction was used for quantification of basic fibroblast growth factor. Within the present study, the highest mean cell proliferation and viability value was recorded in group 2 (normal treated with oil) while the lowest value was recorded in group 3 (periodontitis) with statistically significant difference between groups. The highest mean basic fibroblast growth factor expression was recorded in group 4 (periodontitis treated with oil) and the lowest value was recorded in group 3 (periodontitis) with statistically significant difference between groups demonstrating that sesame oil possess a positive effect on fibroblasts proliferation and function. In conclusion sesame oil is a promising biocompatible anti-oxidant and anti-inflammatory natural extract that can stimulate periodontal ligament fibroblasts proliferation and function through increasing basic fibroblast growth factor gene expression in vitro and therefore it may hold the potential to enhance periodontal regeneration in vivo. |