الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to identify the genetic polymorphisms of some genes that have notable effect on wool quality traits in Egyptian sheep breeds, including staple length (STL), fiber diameter (FD), staple strength (STR) and clean fleece weight (CFW). KRT35 and KRT2.10 (KRT83) genes that code for the keratin intermediate-filament proteins (KRTs) along with microsatellite BFMS were targeted for this investigation. Data from PCR-SSCP assay of the KRT2.10 gene showed one PCR-SSCP pattern, confirmed by RFLP analysis using restriction enzyme (Bse3D I) showing one genotype of KRT2.10 gene with one undigested band at 191 bp. On the other hand, KRT35 using PCR-SSCP analysis revealed three different banding patterns corresponding to three genotypes AA, AB and AC (with frequencies 0.92, 0.02, 0.05 respectively). Sequencing analysis of different KRT35 genotypes revealed that A allele represents substitution of G/A at position 4016, allele B showed substitution of G/A at position 4056, and allele C revealed substitution of C/T at position 4027 |