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العنوان
Tissue culture, phytochemical and biological studies of some medicinal plants belonging to families podocarpaceae and capparaceae /
الناشر
Amira Ali Mohamed Essa Elmahis ,
المؤلف
Amira Ali Mohamed Essa Elmahis
هيئة الاعداد
باحث / Amira Ali Mohamed Essa Elmahis
مشرف / Seham Salah Eldin Elhawary
مشرف / Kamilia Fouly Taha
مشرف / Farid Noshy Kirillos
تاريخ النشر
2018
عدد الصفحات
563 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم الصيدلية
تاريخ الإجازة
8/8/2018
مكان الإجازة
جامعة القاهرة - كلية الصيدلة - Pharmacognosy
الفهرس
Only 14 pages are availabe for public view

from 551

from 551

Abstract

It was interesting to apply different tissue culture techniques for micropropagation of valuable and threatened medicinal plants used in traditional medicine and belonging to two different families and habitats. The study is divided into two parts: Part (I): Forest plants belonging to family podocarpaceae including [podocarpus macrophyllus (Thunb.), podocarpus gracilior (Pilg.), podocarpus elongatus (Aiton) Lʼ Hér. ex Pers]. Part (II): A wild desert plant belonging to family capparacea (capparis spinosa L.). Botanical study and genetic profile of podocarpus species were conducted. In vitro propagation of podocarpus species through tissue culture experiments was done and includes (sterilization, start, micropropagation, subculture, rooting, acclimatization, callus formation, studying the effect of stress factors (Elicitors) on shootlets growth experiments). Phytochemical screening, investigation of the volatile constituents of the leaves of Podocarpus species and identification of the major constituents by GC/MS were also carried out. Investigation of lipoidal matter (saponifiable and unsaponifiable matter) of podocarpus species was done. Also quantitative determination of phenolic content and flavonoids of methanolic extracts of Podocarpus species was done using both colorimetric assay and HPLC analysis. Fingerprint study of different fractions of both leaves and stems of Podocarpus species by HPTLC was carried out where kampferol, apigenin, luteolin and quercetin were identified in different fractions