الفهرس 
Assessment of proliferative capability of stem cells from human exfoliated deciduous teeth cultured in regular culture medium as compared to those cultured in culture medium combined with fibroblast growth factor 6
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Abstract
Background: Stem cells from human exfoliated deciduous teeth (SHED) are considered one of the simplest, most convenient obtained source of adult stem cells. The study aims to perform a molecular assessment of CDK2 and MATK genes{u2019} expression of SHED derived microvesicles after fibroblast growth factor 6 supplementation. Materials and methods: SHED were isolated and cultured from deciduous teeth pulp using collagenase II enzyme, they were characterized morphologically and by Fluorescence-activated cell sorting (FACS). SHED were divided into two groups; control group with no treatment with (FGF-6) and test group with (FGF-6) treatment. At the third passage, SHED proliferation were assessed by using MTT assay. MVs were purified by ultracentrifugation technique and SHED derived microvesicles{u2019} CDK2 and MATK genes expression were assessed by real time polymerase chain reaction. Results: SHED were identified in culture by their spindle fusiform shape. They were positive for CD105 and negative for CD 45. SHED proliferation in the test group supplemented with FGF-6 was significant in comparison with those in the control group without FGF-6 supplementation (P < 0.001). SHED derived MVs{u2019} mRNA from the test group demonstrated a remarkable elevated expression of CDK2 and MATK, (P< 0.002) (P< 0.005) respectively in comparison with those of the control group. Conclusion: The significant effect of FGF-6 on SHED{u2019}s proliferation rate is favorable as the production of large scale number of stem cells is beneficial to stem cell based therapies and tissue engineering fields. SHED derived MVs can be used as molecular biomarkers for SHED proliferation