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Abstract This thesis is concerned with the study of pollen for sixty-six of the woody plants found in the city of New Damietta using light microscope, in addition to the study of some allergenic pollen by separating the total pollen protein content in these samples (SDS-PAGE analysis), and bioinformatics was used to analyze the sequences of some protein sequences that cause allergies. Pollen slides were prepared according to the Erdtman method (1960) and studying the different palynological traits. The results showed a clear variation between the species, but they are very similar to the same plant family. Through this study, it was possible to develop a key by which to identify and differentiate between these plants studied. The proteins in twelve pollen samples were separated and the resulting protein bands in each sample were compared with the other samples and the results were as follows: The appearance of many different protein bands in 9 samples while they did not appear in 3 samples. Phoenix dactylifera recorded seven protein bands with molecular weights of 20, 26, 32, 44, 55, 64, 105 kDa. Five protein bands with molecular weights of 22, 28, 48, 55, 82 kDa in Tecoma stans. Five protein bands with molecular weights of 20, 28, 44,55,72 kDa in Jacaranda acutifolia. Four protein bands with molecular weights of 24,44,55,73 kDa in Ricinus communis. Four protein bands with molecular weights of 32,55,64,95 kDa in Cassia glauca. Two Protein bands in both Acacia ehrenbergiana and Delonix regia have molecular weights of 45,72 and 20,55-250 kDa respectively. |