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العنوان
Some studies on Cryptosporidium species infecting ducks /
الناشر
Marwa Mohammed Eid Khalifa ,
المؤلف
Marwa Mohammed Eid Khalifa
هيئة الاعداد
باحث / Marwa Mohammed Eid Khalifa
مشرف / Ahmed M. Nassar
مشرف / Nisreen E. Mohamoud
مشرف / Azza M. Abdelwahab
تاريخ النشر
2016
عدد الصفحات
137 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
4/3/2017
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Parasitology
الفهرس
Only 14 pages are availabe for public view

from 170

from 170

Abstract

The present work was initiated to study the incidence, morphology, molecular identification, host specificity and life cycle by light and electron microscope of Cryptosporidium spp. infecting ducks. Examination of 915 intestinal fecal samples and 870 bursal impression smears collected from Giza, Cairo and El-Dakhlia governorates by M.Z.N stain revealed that the total incidence of Cryptosporidium spp. was 36.4% and 42% in intestinal and bursal samples respectively. Cairo governorate showed the highest incidence of Cryptosporidium spp. infection (49% and 95%) of the intestinal fecal samples and bursal impression smears respectively. Examination of each of 549 intestinal fecal samples and bursal impression smears of ducks at young age (1W-2M) revealed that 66.5% were infected with Cryptosporidium spp. While the older one ({u02C3}2 to 6 M) were Cryptosporidium spp. negative. Seasonal incidence showed that the highest rate was in winter (69.6% &78.7%) while the lowest one was in autumn was 5.3% & 7.3% of intestinal fecal samples and bursal impression smears respectively. Comparative study between M.Z.N. and ELISA showed that examination of intestinal fecal samples and bursal impression smears of each of 100 Muscovy ducks by M.Z.N. stain revealed that 28 and 33% were Cryptosporidium spp. positive respectively. While ELISA showed that 55% of these ducks had antibodies against Cryptosporidium species. characterization and immunoblotting of Cryptosporidium spp.antigens of ducks revealed that the bands at the MW 68, 38-42, 20 and that lower than 15 KDa considered to be specific for duck strain as they react versus Cryptosporidium positive duck serum only