الفهرس | Only 14 pages are availabe for public view |
Abstract Geothermal water samples collected from Dakhla Oasis, Kharga Oasis, Pharaoh Baths and Ras Sedr hot springs in Egypt were explored for the isolation of industrially efficient thermostable amylase-, protease- and lipase- producers. Of 170 enzymeproducing isolates secured from colonies developed on agar media, 12 superior ones were subjected to morphological characteristics and biochemical profiles. Cells appeared cocci and spiral with the majority as bacilli. Adopting the Diagnostics GN/GP 24 (Ref. 1001, 1002), the tested candidates successfully utilized various substrates as carbon and nitrogen sources besides their abilities to produce a number of exoenzymes. Six potent amylase-, protease- and lipase-producing thermophiles, two for either, were further identified by 16S rRNA gene sequencing. The amylase-producers were identified as Aneurinibacillus thermoaerophilus and Bacillus licheniformis with respective similarity percentages of 99.48 and 100. |