الفهرس 
Evaluation of Snake Venom Detoxification by Using Propolis and Gamma Irradiation
AbstractOnly 14 pages are availabe for public view
 |  | from | 120 |  |  |
| | | from | 120 | | |
Abstract
Snake venom is a fascinating natural blend of proteins and peptides that is rich in variety and
therapeutic potential. Much emphasis has been dedicated to the research of venom detoxification in
various methods, as well as its biological significance. The aim of this study was to evaluate the
anticancer potential of crude CV, irradiated Cerastes cerastes snake venom IRRV and propolis
ethanolic extract &PEE related biological alterations. The study showed that for IRRV, there is a higher
toxicity than CV for A549 and PC3 cell lines but CV-PEE showed a higher toxicity than IRRV and
CV. The toxicity to A549 and PC3 cells was concentration and cell type dependent, in comparison to
controls. The cell cycle analysis were monitored using flow cytometry and mRNA expression levels
of the apoptosis-regulatory genes P53, Casp-3, and BCL-2 were determined by real-time PCR after
treatment with IRRV, CV, PEE, and CV-PEE . Apoptotic genes showed a significant up-regulation of
P53 and Casp-3 genes while the anti-apoptotic gene (Bcl-2) showed a down-regulation. IRRV showed
the highest increase in P543 and Casp-3 and the lowest decrease in Bcl-2 for both PC3 & A549. Also,
induced elevated DNA accumulation in the S phase post- PC3 cell treatment with IRRV and CV, as
well as a significant DNA accumulation at G2/M phase after IRRV treatment of A549 cells. In contrast,
PC3 cells showed a negligible cellular DNA accumulation after PEE treatment. Additionally, the level
of oxidative stress can be measured by assessing the Glutathione reductase GR, Malondialdehyde MDA
and Reactive oxygen species ROS levels. GR was reduced in case of PC3 and A549 cell treated with
IRRV, CV, and PEE compared with its values in untreated cell control. The MDA values in both cells
recorded a significant elevation post IRRV treatment compared to the rest of the treatment regimen and
untreated cell control. Similarly, IRRV and CV-PEE showed obviously higher ROS values than PC3
and A549 cell treatments with CV and PEE. The conclusion, natural products as propolis may be an
alternative way for detoxification of snake venom