الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this study is to evaluate the antitumor activity of three Schiff base complexes in Nano size particles (copper, zinc and mixed copper/zinc complexes NPs) in the treatment of hepatocellular carcinoma induced in rats both invitro and invivo, and the ability of these complexes to ameliorate the antioxidant system, improve the liver functions, histopathological alternations induced by the tumor, reduce proliferation and induce apoptosis in hepatocellular carcinoma. Methodology: Transmission electron microscope: TEM samples for the colloidal suspensions of Cu complex, Zn complex and mixed copper(II)-zinc(II) complex in distilled water were prepared by dropping the colloids onto carbon-coated TEM grids (Carbon coated Cu grids, Ted Pella, Redding, CA, USA) and allowing the liquid carrier to evaporate in air then assaying by a JEOL 1230 transmission electron microscope (120 kV). Animals: 110 healthy male albino rats 8 weeks old (180 - 200 g) were purchased from National Cancer Institute, Cairo, Egypt. Rats were housed in cages at regulated temperature (22- 25 °C). They were kept under good ventilation under a photoperiod of 12-h light/12-h darkness schedule with lights-on from 06:00 to 18:00. They all received a standard laboratory diet (60% ground corn meal, 10% bran, 15% ground beans, 10% corn oil, 3% casein, 1% mineral mixture and 1% vitamins mixture), purchased from Meladco Feed Company (Obour City, Cairo, Egypt) and supplied with water ad libitum throughout the experimental period. |