الفهرس 
Evaluation of tumor associated neutrophils in colorectal carcinoma : Histopathological and immunohistochemical study
AbstractOnly 14 pages are availabe for public view
 |  | from | 186 |  |  |
| | | from | 186 | | |
Abstract
Cancer-related inflammation has been proposed to represent a hallmark of cancer where neutrophils play an important role in tumor progression of colorectal cancer (CRC). CD66b was found to be a reliable marker to identify tumor associated neutrophils (TAN) in colorectal cancer tissues using immunohistochemistry. Objectives:To detect TAN in CRC by immunohistochemistry and correlate their density with the clinicopathologic colorectal carcinoma features. Materials and methods: Tumor biopsies from colectomy specimens of 60 patients with CRC were stained with hematoxylin-eosin for histological evaluation then immunostained with monoclonal antibodies against CD66b which was detected in terms of high and low infiltration by using computer assisted image analysis technique.Results:CD66b showed high infiltration status in 50% of the cases (30/60) where high CD66b infiltration status showed statistically significant correlation with the longest tumor dimension (p-value 0.01), lymph node status (p-value <0.001), TNM stage grouping (p-value 0.005), Modified Dukes’ classification (p-value 0.002) and perineural invasion (p-value 0.03). However, there was no statistically significant correlation between CD66b infiltration status and age, gender, tumor site, tumor histological type, tumor histological grade, extent of primary tumor, distant metastasis or lympho-vascular embolization.Conclusion: TAN is recognized as a potential protective factor against colorectal cancer and might be used as an independent prognostic marker for colorectal carcinoma as early stages of colorectal cancer are highly associated with the abundance of neutrophils.