الفهرس | Only 14 pages are availabe for public view |
Abstract In the present study, the electroporation technique was used to make reversible pores through liposomes membrane and subsequently effective loading of the drug to liposomes. The effect of electroporation on the physical characteristics of diclofenac sodium (DS), ascorbic acid, and rhodamine B (Rh-B). loaded 1,2-dipalmitoyl-Sn-glycero-3-phosphocholine (DPPC) liposome was studied. Ascorbic acid and DS were used as examples of loaded drugs, and Rh-B fluorescence dye as labeling to visualize the entrapping liposomes. Samples of DS, ascorbic acid, and Rh-B were divided into three groups. group one represents liposome vesicles incubated with DS, group two was liposomes with ascorbic acid and, group three was liposomes with Rh-B. Liposomes were prepared using the thin-film hydration method. The three groups were subjected to electroporation protocol. Samples in electroporation cuvette, were exposed to different field strengths (200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000 and, 3000 V/ 0.2 cm) at pulses 30 pulses, 60 pulses and, 90 pulses and pulse duration 4 milliseconds. After application of the electroporation protocols, liposomes were separated from unentrapped DS, ascorbic acid, and dye respectively by centrifugation. Physical characteristics of liposomes were examined using Transmission Electron Microscopy (TEM),Confocal Laser Scanning Microscopy (CLSM), and Particle size analysis. Liposomes entrapping ascorbic acid with high efficiency at the values of 900 V/ 0.2 cm with 60 pulses, 800 and 1000 V/ 0.2 cm at 90 pulses. While DS was entrapped at 800 V/ 0.2 cm and 90 pulses. Rh-B was entrapped at 500 and 1000 V/ 0.2 cm at 30 pulses. Based on the present study, entrapping drugs into liposomes can be controlled by electroporation technique. Optimum entrapping occurred at 800 V/0.2 cm and 90 pulses for ascorbic acid and DS and at 900 V/0.2 cm and 30 pulses for Rh-B. |