الفهرس | Only 14 pages are availabe for public view |
Abstract Background/objective: Graft-versus-host disease (GVHD) is currently one of the serious complications after allogeneic hematopoietic stem cell transplantation (AHSCT). Many studies have shown the potential role of non-human leukocyte antigen (non-HLA) gene polymorphism with the incidence of GVHD. In this study, we analyzed the impact of single nucleotide polymorphism (SNP) within the promoter region of the interleukin-10 (IL-10) at positions (-1082, -819, and -592), and IL-6 (- 174) on the development of GVHD and overall survival (OS) following pediatric AHSCT. Method: In this retrospective analysis, we included 94 patients with different hematological malignancies who received BM (n=80) and PB (n=14) grafts from their HLA-matched related donors. Most patients (n=85) were treated with myeloablative conditioning (MAC), whereas the remaining patients (n=9) received reduced-intensity conditioning (RIC). We used polymerase chain reaction sequence-specific primer (PCR-SSP) to analyze the SNPs of IL-10 and IL-6 in 94 patients and 92 donors. Results: In univariate analysis, we did not find a statistically significant association between the IL-10 genotypes with the incidence of acute or chronic GVHD. Donor IL-6 GG homozygous genotype was found to be significantly associated with the development of cGVHD (p=0.028). The result of donor IL-6 GG together with other factors known to affect the risk of aGVHD and cGVHD were subjected to multivariate logistic-cox regression analysis. This analysis confirmed the association of donor IL-6 GG homozygous genotype to the risk cGVHD (p=0.049). No significant association was found between IL-10 and IL-6 gene polymorphisms and overall survival (OS). Conclusion: Our work identified the Donor IL6-174 GG genotype of pediatric AHSCT as a risk factor for the development of cGVHD. |