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Abstract
The aim of this study was to reach a well defined protocol protocol for in vitro propagation of Gardenia jasminoides ellis, cordline fruticosa atom and spathiphyllum mauna Loa. For, G. jasminoides, the best survival rate (100%) was found by using 1.5 % NaOCl for 25 min with zero% contamination and mortality. NAA at 4.0 mg / l during establishment stage, resulted in the highest shoot length and the highest number of leaves. For multiplication stage, 5mg / l BA was produced the highest number of shoots. At rooting stage, 3.0 mg / l IBA plus activated charcoal resulted the highest shootlet length and number of leaves. While, MS medium with activated charcoal contained 5.0 mg / l IBA produced the greatest number and length of roots. All plantlets acclimatized at pots containing 1 : 2 (v / v) peatmoss and sand. As for. Cordyline fruticosa, the highest survival percentage (100%) and the lowest mortality and contamination percentages were found at 2.0 % NaOCl for 25 min. At establishment stage, the highest number of shoots, shoot length and number of leaves were at full salt - strength of MS medium free hormon