الفهرس | Only 14 pages are availabe for public view |
Abstract Objective: This work was conducted to investigate the cardioprotective effect of grape seed extract and L-carnitine against doxorubicin-induced cardiotoxicity in rats. Methods: A total of 60 male albino rats. were divided into 6 groups, group I: rats served as the control group and received 0.5 ml/kg b.wt. of normal saline 0.9% NaCl solution for the whole experimental period (35 days), group II: given grape seed extract (GSE) (100 mg/kg b.wt., daily) for the whole experimental period, group III: given L-carnitine (L-CA) (150mg/kg b.wt., twice per week) for the whole experimental period, group IV: given doxorubicin (DOX) i.p. on day 28 of the experiment to induce cardiotoxicity (10mg/kg b.wt.), group V: given combination of GSE +DOX, group VI: given combination of L-CA +DOX. On days 29 and 35 of the experiment. the following parameters were measured: body weight, heart weight, ECG, hematological, some serum biochemical parameters, lipid profile (TC, TAG, HDL-c, LDL-c, VLDL-c), cardiac injury markers (CK-MB, LDH, AST, cTnI, and NT-proBNP), oxidative stress markers and antioxidant enzymes (GSH, CAT, TAC, TOC, MDA, TNO, and LOOH), inflammatory markers and cytokine analysis (IL-1 β, TNF-α, MPO, and NF-κB), histopathological examination of heart was also performed. Results: The results showed that GSE and L-CA significantly ameliorated the ECG abnormalities, the levels of serum cardiac markers, oxidative stress markers and antioxidant enzymes, and inflammatory, and cytokine analysis that deteriorated by DOX. Histopathological examination of heart sections of rats pre-treated with GSE and L-CA showed alleviation of histological degenerative changes caused by DOX. Conclusion: The results suggested that the GSE and L-CA induce potent cardioprotective and antioxidant effects in DOX-induced cardiotoxicity in rats. |