الفهرس | Only 14 pages are availabe for public view |
Abstract One of the major problems of avian infectious bronchitis virus (IBV) is the frequent emergence of new variants. In the present study, 18 suspected farms from broiler chicken farms were collected during 2019 to august 2022 from different governorates in Egypt. These farms showed sinusitis, pharyngitis and respiratory illness. Eleven suspected farms were positive for IBV with real time RT-PCR. The samples were homogenised, prepared and inoculated in specific pathogen free (SPF) embryos for three blind passages and examined with PCR. The hypervariable region 1 and 2 of spike protein (SP1) was amplified and sequenced to study the genetic diversity between virus isolates. Ten isolates were selected and the phylogeny was carried out between selected isolates and other IBV from the Middle East in gene bank. The S1 gene was clustered into two major groups , the first group contain only one virus belong classical strain GI-1 and the second group contain genotype GI-23 (variant II), with nine viruses occurring in two subgroups, first subgroup GI-23.2.1, contains 8 viruses and second subgroup contains one viruse belong to genotype GI-23.2.2 |