الفهرس | Only 14 pages are availabe for public view |
Abstract Aim of the Study: This study was carried out to clarify the effect of combining fluoride or CPP-ACPF with self-assembly peptides in comparison to the remineralizing potential of self-assembly peptides on their own. Null Hypothesis Tested: First, there is no difference in the remineralizing potential between self-assembly peptides, fluorides, and CPP-ACPF. Second, the combination between fluoride, CPP-ACPF and self-assembly peptides will not have any effect on remineralization of artificial carious lesions. Materials: 1) Demineralizing Solution. 2) Artificial Saliva. 3) Remineralizing Agents: Bifluorid 10 (fluoride-based varnish), MI Varnish (CPPACPF-based varnish), and Curodont Repair (self-assembly peptide-based remineralizing agent). Methodology: 1) Teeth selection: Sixty sound freshly extracted molar teeth were collected and inspected under a stereomicroscope to exclude any enamel defects, and they were cleaned. 2) Grouping: Specimens were randomly allocated into two equal groups of 30 specimens each according to use of the biomimetic material: a. Control group (B0): specimens did not receive biomimetic material, b. Test group (B1): specimens were treated with Curodont Repair. Each group was further subdivided into 3 subgroups 10 specimens each according to the remineralizing material used; either artificial saliva, fluoride (Bifluorid 10), or CPPACPF (MI Varnish). Summary - 72 - 3) Specimens Preparation: Molars were sectioned horizontally 2 mm below the cemento-enamel junction; crowns then were embedded in cylindrical acrylic resin molds with the buccal surfaces facing upwards. The buccal surfaces were flattened using finishing and polishing disks. After that, a 4 × 4 mm window was created on the buccal surface of each specimen and the whole remaining enamel surface was covered by an acid resistant nail varnish |