الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 272 |  |  |
| | | from | 272 | | |
Abstract
This study aimed to investigate the antidiabetic activity of Matricaria aurea
(Golden Chamomile, MA) in a type 2 diabetic rat model, then elucidate the
action mechanisms by using methods from molecular biology, bioinformatics,
and network pharmacology. Our results demonstrated that only the polar
ethanolic extract of MA had remarkable antidiabetic activity, while less polar
extracts did not show any promising activity. Furthermore, it improved
dyslipidemia, insulin resistance status, ALT, and AST levels. LC-MS/MS
analysis of MA ethanolic extract identified 62 compounds, including the
popular chamomile flavonoids apigenin and luteolin, other flavonoids and
their glycosides, coumarin derivatives, and phenolic acids. Bioinformatics and
network pharmacology analysis revealed that 46 phytochemicals in MA can
modulate many proteins associated with inflammatory pathways, oxidative
stress pathways, and energy-sensing/endocrine/metabolic pathways. q-
RTPCR gene expression analysis showed that MA ethanolic extract was able
to significantly upregulate PIK3R1 and downregulate IL1B, PCK1, and
MIR29A. Moreover, the activity of the antioxidant hub enzymes was
substantially increased. Molecular docking scores were also consistent with
Author Yassin Ismail Attia Abdelhamid
Title Evaluation of the Antidiabetic Effect of Matricaria aurea
(loefl.) in Streptozotocin-Induced Diabetic Rats
Faculty Faculty Of Pharmacy, Suez Canal university
Department Department of Biochemistry
Location Ismailia, Egypt
Degree M.Sc. in Pharmaceutical Sciences (Biochemistry)
Date 25/7/2023
Language English
Supervision
committee
Prof. Dina Mohamed Abo-Elmatty, Associate Prof. Dina
Mostafa Fahmy, and Dr. Samy Mohamed Saleh
the networks’ predictions. Based on experimental and computational analysis,
this study revealed for the first time that MA exerted antidiabetic action via
simultaneous modulation of multiple targets and pathways, including
inflammatory pathways, oxidative stress pathways, and energysensing/
endocrine/metabolic pathways.
Key words: Matricaria aurea (MA), Diabetes, Network pharmacology, LCMS/
MS, metabolic pathways, Oxidative stress, inflammatory pathways.