الفهرس 
Materials and methodsOnly 14 pages are availabe for public view
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Abstract
The three genotypes Sakha 53, Giza 102, and Par -1671-1 were used as sensitive, moderate and high in salt tolerance, respectively for in vitro study. Plants of each genotype were germinated under sterilized environments; hypocotyls and cotyledons were dissected out from 10 days old seedlings. And the cultured on three different mediums (M1, M2 and M3) for callus induction Calli of the three different genotypes were sub cultured on M2 medium using different levels of NaCl (0, 50, 75, 100, 125 and 175 mM). The survived calli under 100 mM and upward were used for further sub culturing under (100,125, 150, 175 and 200 mM) NaCL concentrations for obtaining more tolerant lines. shoot regeneration the healthy calli were selected and cultured again where it was started in two or three weeks on two MS medium The survived shoots were rooted on two mediums MS medium supplemented with 1 mg/l BA or hormone free medium healthy calli were cultured for shooting and rooting the good healthy produced plantlets were acclimatized and transplanted in pots in the greenhouse. The produced plants were grown until maturity and the seeds of each plant were harvested. The seeds of each plant were considered as an independent line and 17 lines were obtained (7, 5 and 5 lines from Giza102, Sakha 53 and Par-1671-1genotypes, respectively). The produced lines via somaclonal variation were used in a comparative study with their donor genotypes under two treatments of salinity (control and salty stress) in a pot experiments. The field study showed that all studied parameters were affected by increasing salinity in the soil e.g. days to emergence, plant height, 100 seed weight, seed yield, oil content and protein.