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Abstract i ! 1. The Yemen Arab Republic lies between latitudes lao 40t, 17° a6’ north and longitude 4ao JO’, 46° Jl’ east and bordered by Saudi Arabia in north, the Peoples Democratic Republic of Yemen in the south and south east and Red Sea in the west. However, the eastern botmdaries are rather il1defined. 2. Yemen i: ~ocated in the northern stretchs of the tropical zone with Vwo rainfall maxima: one in April- May, the other in July-September and a long dry, almost rainless period of four to five months during the late autumn and winter. J. An ecological survey of a part of Sanata-Taiz road waB made. The plant cover may be classified in relation to land-form into: (a) Vegetation of table land, (b) Vegetation of rocky hill countries or ridges and (c) Vegetation of slopes of mountains. The vegetation of various geomorphological units was subdivided into communities. The most prominant feature observed was the repetition of the plant communities with the repetition of habitats. The phytochemical screening of ten species representing ------- --- --------------- r\ rI: ”It ’~. ’j \i- Tj’,L - 219 - different ~ami1ies revealed variations in their constituenta according to the characteristics of their families. ~. The species concerned, Rumex nervosus, inhabits various habitats ranging from moist land to vertically cutcliff mainly of barren rock. Three different habitats namely: (a) level habitat, (b) slope habitat, and (c) 5umara mountain at elevation of )000 m were selected for the study of the behaviour of Rumex nervosuS. The behaviour of the root system was described in the different habitats which may be vertical, horizontal, forming rhizomatous sucker, or turn up and screw according to the nature o~ the substratum. 6. 50il texture, water holding capacity, moisture equivalent are among the physical factors affecting the life of the plant. The pH of the soil was found to be moderate alkaline (8.~to 8.8).The electrical conductivity for all samples were less than 1 mm hosl cm indicating that there was no development for alkalinity or salinity in the soil supporting Rumex nervosus in the three different habitats. The organic matter content, calcium carbonate content and soluble cations and anions were also determined. The soil and plant moisture contents showed monthly , - aao •• fluctuations owing to the seasona~ity of rainfall and other climatic factors. 7. With variation in time of flowering and fruiting, the phenological behaviour of the plant was the same in the three different habitats. The plant does not pass a pronounced dormancy. The enlarged perianth around the fruits of the plant • was characterised by its pink colour which apparently started to appear after fertilization. The colour intensity uhich depends upon the degree of exposure to direct light was attributed to the presence 01’ anthocyanin pigments. 8. The study of seed germination under the influence of some factors revealed that the water extract obtained from soaking of seeds with the two enlarged sheathes inhibited the germination of wheat grains. This might be due ’to ei.’ther’to i;he etfect of ’tN’nins on enzymatic activity, or to the presence of growth inhibiting substances in the outermost sheath of the seed. 9. The study of the community type of Rumex nervosus was carried out by means of studying the analytical as well as the synthetic characters. The similarity between the communities in the three different habitats • -------~. r:: ”,. 1’;1 - 221 - was ca1cu1a~ed according ~o simi1ari~y quo~ien~ (Sorensen, 19~8). 10. Concerning the phy~ochemica1 inves~iga~ion of ~he p1an~ i~ was fO\L~d~ha~ ~he va1ues ~f ~o~a1 ash con~en~, wa~er so1ub1e ash, acid inso1ub1e ash, crude fibre con~en~ and ascrobic acid con~en~ were higher in ~he shoo~s ~han in ~he frui~s in bo~h habi~a~s name~y Sumara moun~ain and ~ab1e 1and. Frui~s showed higher va1ues of tota1 ni~rogen con~en~, crude pro~ein con- ~ent and to~a1 1ipid content than shoots in the bo~h mentioned habi~ats. 11. The data of extraction with successive se1ective organic solvents revea1ed ~hat the fruits yie1ded a higher percentage of ~he tota1 residue being ~8.07%. 12. The pre1iminary phytochemica1 screening of each p1ant organ inc1uded the tests for carbohydrates and/or ~Ii Ii\ , I, g1ycosides, stero1es, terpenes, tannins, saponins, f1avonoids, a1ka1oids, muci1age, resin, ch10rides and su1phates. The resu1ts obtained indicated tha~ terpenes and saponins were absent in the fruits whi1e -the a1ka1oids were absent in both shoots and fruits. 1). Chromatographic ana1ysis of mucilage hydro1ysates revea1ed the presence of D_g1ucose and D-g1ucouronic acid in both fruits and shoots. . By chromatographic analysis of free and combined sugars. glucose, galactose, sucrose and tructose were the ,. deteoted free sugars while gluoose, galactose, fruc- ,!, tose, arabinose and rhamnose were the deteoted combined sugars. j 15. The tree and protein amino acids w~re identified chromatographically and the results revealed the presence of twelve free amino acids and eleven protein amino acids namely, histidine,arginine, glycine, iI glutamic acid, threonine, tryptophane, isoleucine, ’\ alanine, valine, leucine, and lycine. 16. Alcoholic extracts of both fruits and shoots contained citric, tartaric, and succinic acids. 17. The percentage of gallotannins in fruits and shoots of Rumex nervosus were tound to be 10.45% and 15.60% respectively. Chromatographic analysi~ ot acid hydrolysates ot gallotannins indicated that it consisted of gallic acid and glucose. 18. The flavonoid contents were studied using paper, thin \ I, layer, and column chromatographic techniques which revealed the presence of rutin glycosides. By means .,I. ( ot acid hydrolysis (5% H2S0~) ot the separated rutin tollowed by ethyl acetate extraction, the sugar part , I -,’-- ”---- ---------- - aa, - (glycone) was identified as glucose and rhamnose, while t~e nonsugar part (aglycone) was identioal to quercetin. Some properties of the separated flavonoids were studied. 19. Anthocyanin of the coloured fruits of the plant was . extracted by l~ HC1 in methanol. The method was based on partial purification of the extract using paper • chromatographic technique. The aglycone part was isolated from glycone one by means of hydrolysis at 97°C followed by extraction with ethyl acetate. 20. The saponins present in the shoot of the plant were extracted by means of 80% ethyl alcohol and investigated chromatographically and by means of IR spectrometry. On hydrolysis of the isolated saponin, xylose and galactose were detected in sugar moiety. The aglycone part of saponin had a m.p. of 162°C. The IR spectrum and other properties were studied. 21. The physical and chemical properties of the oil in the fruits and shoots of Rumex nervosuS were studied. Thin layer chromatography of the unsaponifiable fraotion of the oil revealed the presence of four steroidal compounds. The main sterol was isolated by meanS of column chromatography and crystallized from chloroformmethanol. The isolated crys~al. had a melting point ’-----_. - --- of 1)90C which did not show any depression upon mixing With an authentic sample of B-sitosterol. Chromatographic separation of the saponifiable fraction of the oil revealed the presence of the unsaturated fatty acid, linolenic acid, while the saturated ones wore identical with stearic and palmitic acids. 22. A crystalline principle was isolated from petroleum ether extract by using two different methods. It was soluble in ether, chloroform and ethyl acetate with m.p. 7)-7qOC. The UV and IR spectra were’also determined. The microanalysis of the isolated compound indicated that it consisted of C, 8).); H, 5.5, 0, 11.2 with an emp1ri.cal formula of C10H80. SuJ.phur, mtrogen and halogens were completely absent. |